Western Blot analysis of B7-H3 (CD276) in extracts of various cell lines using B7-H3 Polyclonal Antibody (Product # PA5-87829) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supplier Page from Thermo Fisher Scientific for B7-H3 Antibody